It's known that Peter Pan needed fairy dust to fly. He used it on Wendy, John, and Michael so they could fly with him to Neverland. (But in the books, there's no indication that the Lost Boys can fly -- if I misremember, please correct me!)
If a one-time dose of fairy dust was enough to let someone fly from then on, one would think we'd see the Lost Boys fly, since they would have had to fly to Neverland in the first place. (But it's also possible that, like the Indians and mermaids and pirates, they were native to Neverland all along.)
Does Peter (or anyone else) need repeated doses of fairy dust to continue flying, or is one dose enough to last for a lifetime?