At the end of The Last Crusade, the Austrian woman attempted to take the Holy Grail beyond the great seal at the entrance of the temple.
In doing so, she caused the temple to quake and the Grail fell into a chasm that was created.
One of the last scenes in the temple shows the last Templar Knight waving bye to Indiana as the escaped.
Was the grail completely lost to the world now? Or did the Knight collect it and reset the temple for the next person to face the tests and discover the grail?