"Hit the road, Jack" is a very popular song and used in plenty of films and TV.
But that list doesn't include any SciFi or fantasy movies or TV. That's not conclusive - I know the Wikipedia list is incomplete because it didn't include my favorite use, from "Failure to Launch". Despite its name it's a romcom, not an astronaut movie so it doesn't count.
Was it ever used in a SciFi or fantasy movie or TV at all? If so, which one was the first one to use it?