After getting bitten by the radioactive spider, does Peter still ride the bus to school, or does he start web-slinging to get there? Is this ever established in the comics? (While I am interested in any depictions of such travel, I will further stipulate that I am primarily interested in the main universe of Earth-616, not the alternate universes or Ultimates, nor any of the non-Peter Spider-men, nor any version where he is no longer in high school.)
In Peter Parker, The Spectacular Spider-Man: The Vulture Is a Bird of Prey, Peter wakes up late and can't get on the bus like he wanted to:
He ends up webslinging onto the bus roof as Spiderman but then gets knocked off by a branch, forcing him to have to find his way to school:
At this point he is in college and the bus is a double decker public one, not a school bus. He ends up being late for class.