No. It can only be used on humans.
Homenum Revelio is a spell that detects the presence of human beings (not animals).
"But before we go any further, I think we'd better check", whispered Hermione, and she raised her wand and said "Homenum revelio".
"Well, you've just had a big shock," said Ron kindly. "What was that supposed to do?"
"It did what I meant it to do!" said Hermione rather crossly. "That was a spell to reveal human presence, and there's nobody here except us!"
(Deathly Hallows, Chapter 9, A Place to Hide).
So it detects humans, not animals. This is confirmed by J.K. Rowling in the linked-to question.
Dumbledore, who could perform magic without needing to say the incantation aloud was using 'Homenum revelio' - the human-presence-revealing spell Hermione makes use of in Deathly Hallows.
(Bloomsbury Live Chat).
Now Animagi are clearly humans on one level. A person who transforms into their Animagi form retains their memories, intellect, agency and identity. But the important thing about Animagi is that the person becomes an animal. It's a holistic transformation, not a disguise.
He hardly heard what Professor McGonagall was telling them about Animagi (wizards who could transform at will into animals...
(Prisoner of Azkaban, Chapter 6, Talons and Tealeaves).
Once transformed, the Animagi is an animal, not a human. So the Homenum Revelio spell would have no effect on them, and certainly wouldn't force them to transform back into their human form.
There is a spell that can do this. It's the one that Lupin and Black use on Pettigrew to force him to transform in Prisoner of Azkaban. However, this spell is never named (as Lupin and Black never make an incantation of any kind) so we can't definitively say what it is. I think that we can rule out Homenum Revelio, though. It doesn't affect animals and it isn't used for transformation (just for identification).