I am often riddled by this question when I'm reading books about vampires. It also occurs when I'm watching a movie about vampires too.

Can a vampire impregnate a human in Twilight?

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    Only if the vampire is smoking hot and sparkles in the sun. Otherwise, no. – Adamant Nov 15 '17 at 9:09
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    But I’d limit this question to Twilight, if I were you (is that what you meant by the tag?) It’s a little broad for fiction in general. It has a really easy answer in Twilight, too. – Adamant Nov 15 '17 at 9:10
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    Different canons have different kinds of vampires. It's like asking if space aliens are allergic to green rocks. – Kreiri Nov 15 '17 at 9:11
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    I've edited your question to be only about Twilight since that's what you tagged it with, and it would otherwise be closed. If that's not what you meant, please edit to clarify. – user58 Nov 15 '17 at 10:05

The answer is a firm yes.

Everything inside me came undone as I stared at the tiny porcelain face of the half-vampire, half-human baby.
Twilight, Breaking Dawn

And from the film of Bella pregnant with Edward's baby.

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    How is babby formed? How vampire get pragnent? – Adamant Nov 15 '17 at 10:29

As evidence by the fourth book, yes, vampires in twilight can impregnate a human. typically it doesn't happen, as most* vampires would lose control and end up draining said human of blood during the interaction, or break them. This can only happen in Male vampire with female human relationships, as a female vampire is in stasis, and no longer has monthly fluctuations, whereas a male vampire, it seems, still has reproductive ability.

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