In a word, no. We see at least one non-Ori ascended Ancient (Morgan le Fay) in the sequel film, The Ark of Truth. She explains that it's difficult to act openly because other ascended beings might prevent her from doing so.
DANIEL: Merlin died. He didn't ascend. His consciousness left me. It's—it's gone. The Ancients must be able to see through your disguise. I know I can, I just don't know why you think it's necessary.
MORGAN LE FAY: You were once ascended. You know how complicated it can be. Interfering with the lower planes is not allowed.
Admittedly, it's possible that she was hiding in the Pegasus Galaxy when Merlin's weapon activated and that she's referring to other ascended beings that are preventing her from acting, but you would imagine that she would have mentioned it if all of the Milky Way ascended had also died when the weapon activated.
Purely for the record, the item that Vala identifies as being the timing crystal is the final piece that she inserts into the Sangraal to activate it.
Note also that the fact that the Ori Mothership doesn't come back through the wormhole at a later date would strongly suggest that the Sangraal exploded or damaged the ship in a way that prevented its further use and the recovery of the Sangraal itself.