The Hufflepuff welcome letter on Pottermore makes a claim about their Dark wizard turnout:
However, it’s true that Hufflepuff is a bit lacking in one area. We’ve produced the fewest Dark wizards of any house in this school. Of course, you’d expect Slytherin to churn out evil-doers, seeing as they’ve never heard of fair play and prefer cheating over hard work any day, but even Gryffindor (the house we get on best with) has produced a few dodgy characters.
Of course, take that with a pinch of salt, since it comes from a Hufflepuff prefect.
That it says “the fewest” might suggest that they have turned out a non-zero number of Dark wizards/witches in their history, but less than the other houses (so the answer to the original question would be yes). But I don’t know of any canon examples that back up that interpretation.
It’s also possible that this Prefect doesn’t know of any instances of Dark wizards in Hufflepuff, but doesn’t want to say “no Dark wizards” in case there are some that they don’t know about.
Comments in the Slytherin welcome letter also hint at Dark wizards across all three houses, but again fails to cite specific instances:
I’m not denying that we’ve produced our share of Dark wizards, but so have the other three houses – they just don’t like admitting it.
But again, that comes with the bias of being written by a Slytherin prefect.
Overall, I’d say it’s pretty likely that Hufflepuff have turned out some Dark wizards and witches, but they probably pale in comparison to the number and notoriety of those turned out by Slytherin, hence they don’t tend to get mentioned much.