The opening sequence of Mass Effect 2 features Wilson, one of the specialists assigned to revive the dead Commander Shepard, betraying the team and trying to kill them all with the station's mechs. However, his motivations aren't revealed, and the other characters quickly move on without questioning his actions.
According to Wilson's entry in the Mass Effect wiki, recoverable logs reveal Wilson's growing dissatisfaction with the project, and with Miranda in particular. Then, files from the Shadow Broker DLC reveal that Wilson was working for the Shadow Broker.
However, this still doesn't seem to answer the question. Why would the Shadow Broker want to stop Project Lazarus? The Shadow Broker volunteered information to Shepard during Mass Effect 1, indicating that he understood Shepard's value in fighting the Reapers, and we know from the DLC that the Shadow Broker was just as aware of the coming invasion as the Illusive Man was. It would make sense for an information broker to steal data about reviving the dead, and he was always in competition with the Illusive Man, but trying to destroy the station just as Shepard was ready to wake up seems like odd timing, and assigning a biologist to slaughter dozens of people seems like a weak play for someone as powerful as the Shadow Broker.
Are we to believe that Wilson acted alone in attacking Project Lazarus, a disgruntled employee who was already a corporate spy just taking it one step further? Or were Wilson's actions taken at the instruction of the Shadow Broker, and if so, why would the Shadow Broker try to destroy such a valuable asset for the coming war?