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I was debating with a friend about this! Is Neo genetically different from others plugged in the Matrix, as in genetically engineered to be the 'One'? Or is he a normal human and it's all software that gives him powers.

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According to the Architect, Neo is engineered but not genetically:

The function of the One is now to return to the Source, allowing a temporary dissemination of the code you carry, reinserting the prime program.

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Your 5 predecessors were, by design, based on a similar predication - a contingent affirmation that was meant to create a profound attachment to the rest of your species, facilitating the function of the One.

The Matrix Reloaded

He is designed to perform the function of the One, which includes a temporary dissemination of the code carried by the One. Genes are a type of code, but the Architect is probably referring to machine code since Neo's code is disseminated when he returns to the Source (a machine).

In other words, he's a normal human who has been given special code (software) by the machines to carry out the function of the One.

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