Assuming you want to discount anything that's Firefly-related, the winner is Con Man, Wash [ahem, sorry] Alan Tudyk's pet project. It has six of the main actors, although not all in the same episodes.
- Alan Tudyk
- Nathan Fillion
- Sean Maher
- Jewel Staite
- Summer Glau
- Gina Torres
As of May 2016, second place goes to Castle, with 5 veterans:
- Nathan Fillion
- Adam Baldwin
- Gina Torres
- Summer Glau
- Jewel Staite
Third place is a tie between CSI (oddly enough) and Justice League.
CSI: Crime Scene Investigation has had the following main Firefly actors, although definitely not in the same episodes. (Which means that the show doesn't come up if you do a "credited with" search on IMDB.)
- Ron Glass
- Summer Glau
- Alan Tudyk
- Gina Torres
The Justice League animated TV series has also had four of our actors doing voices:
- Adam Baldwin
- Morena Baccarin
- Gina Torres
- Nathan Fillion
Honorable mention: Buffy-verse has had four actors, but not in the same series. Nathan was in Buffy itself, and Summer, Adam, and Gina all appeared in the spin-off Angel.
5th place is a three-way tie between the aforementioned Angel and the video games Halo 3 and Halo 3: ODST, which both had Adam, Alan, and Nathan.
Looking at it from the actor's perspective, Adam Baldwin has shared the screen most often with his erstwhile co-stars, with 19 of his shows having at least one other Firefly alum. The person who has worked on the same shows as her co-stars the least is Jewel, with only 4 shows: Con Man, The X Files (Adam), Warehouse 13 (Sean), and Castle. The numbers are:
- Adam 19
- Alan 18
- Gina 13
- Jewel 4
- Morena 8
- Nathan 16
- Ron 5
- Sean 9
- Summer 11